Relative Quantitation of Protein Abundance Relative Quantitation of Posttranslational Modifications
نویسندگان
چکیده
Sample Preparation for the Relative Quantitation of Protein Abundance Sample Preparation for Monitoring Components of a Protein Complex Sample Preparation for Relative Quantitation of Posttranslational Modifications Sample Preparation for the Identification of Tyrosine Kinase Substrates Electrophoresis and Silver Staining of Polyacrylamide Gels In-Gel Trypsin Digestion Reversed Phase Column Preparation LC-MS/MS Analysis
منابع مشابه
iTRAQ-based quantitative analysis of protein mixtures with large fold change and dynamic range.
Quantitation of changes in protein abundance is key to understanding the alterations that biological systems undergo and to discovering novel biomarkers. Currently, HPLC-MS/MS can be used to quantify changes in protein expression levels [Ong, S. E. and Mann, M., Nat. Chem. Biol. 2005, 1, 252-262]. Nevertheless, quantitative analysis of protein mixtures by HPLC-MS/MS is still hampered by the wid...
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Recent advances in proteomics have become an indispensable tool for a fast, precise and sensitive analysis of proteins in complex biological samples at both, qualitative and quantitative level. In this study, a label-free quantitative proteomic methodology has been optimised for the relative quantitation of proteins extracted from raw pork meat. So, after the separation of proteins by one-dimen...
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iTRAQ (isobaric tags for relative or absolute quantitation) is a mass spectrometry technology that allows quantitative comparison of protein abundance by measuring peak intensities of reporter ions released from iTRAQ-tagged peptides by fragmentation during MS/MS. However, current data analysis techniques for iTRAQ struggle to report reliable relative protein abundance estimates and suffer with...
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